Object_table = Per_Object = Database Columns = Specify the database column names that contain unique IDs for images and objects (and optionally tables). #Mon Jul 12 14:55:00 2010 = CellProfiler Analyst 2.0 properties file = Database Info =ĭb_passwd = a99l3Core = Database Tables = SELECT Nuclei_Location_Center_X, Nuclei_Location_Center_Y FROM Per_Object WHERE (ImageNumber=2 AND ObjectNumber=1) Opening image: R:\CellProfilerWork\cellProfiler_examples\CirculatingTumourCells\biocept_website\CTC_RobustProstateBreastOvarian.jpg SELECT Image_PathName_CTC, Image_FileName_CTC FROM Per_Image WHERE ImageNumber IN (1) SELECT Nuclei_Location_Center_X, Nuclei_Location_Center_Y FROM Per_Object WHERE (ImageNumber=1 AND ObjectNumber=1) SELECT Nuclei_Location_Center_X, Nuclei_Location_Center_Y FROM Per_Object WHERE (ImageNumber=2 AND ObjectNumber=3) Opening image: R:\CellProfilerWork\cellProfiler_examples\CirculatingTumourCells\biocept_website\CTC_image_preview.jpgįetched 5 random cells from whole experiment SELECT Image_PathName_CTC, Image_FileName_CTC FROM Per_Image WHERE ImageNumber IN (2) Connected to database: CTCtest1 as SELECT Nuclei_Location_Center_X, Nuclei_Location_Center_Y FROM Per_Object WHERE (ImageNumber=2 AND ObjectNumber=2) SELECT ObjectNumber FROM Per_Object WHERE ImageNumber=2 LIMIT 0,1 SELECT ObjectNumber FROM Per_Object WHERE ImageNumber=1 LIMIT 0,1 SELECT ObjectNumber FROM Per_Object WHERE ImageNumber=2 LIMIT 2,1 SELECT ObjectNumber FROM Per_Object WHERE ImageNumber=2 LIMIT 1,1 SELECT Per_Image.ImageNumber FROM Per_Image SELECT Per_Object.ImageNumber, COUNT(Per_Object.ObjectNumber) FROM Per_Object GROUP BY Per_Object.ImageNumber SELECT ImageNumber FROM Per_Image GROUP BY ImageNumber
TableNumber column was found indexed in your object table but not your image table. Connected to database: CTCtest1 as SHOW INDEX FROM Per_Image PROPERTIES WARNING (plate_type): Field is required for plate map viewer.Ĭould not import cpfigure, will not display Margin vs. PROPERTIES WARNING (well_id): Field is required for plate map viewer. PROPERTIES WARNING (plate_id): Field is required for plate map viewer. PROPERTIES: Using default tile_buffer_size=1 PROPERTIES: Using default image_buffer_size=1 PROPERTIES WARNING (channels_per_image): No value(s) specified. I would be grateful if you could let me know what is wrong here… R:\CellProfilerWork\cellProfiler_examples\CirculatingTumourCells\biocept_website\CTC_RobustProstateBreastOvarian.jpg I uploaded my CellProfiler file and Screen Snapshot für both here, Images are too big to be uploaded.I have processed some test image files in CellProfiler, and created a properties file however when I read this into CellProfilerAnalyst I seem to be getting an problem where the filepath is concatenated onto the filename there is a extra backslash – I think this is providing problems when the file access is attempted.
#Cellprofiler analyst image registration plus#
My image file name plus path name is more than 64, but the maximal character numbers are for column name limited, or? I used CellProfiler 3.1.9 and Analyst 3.0.0, and there are no empty space in my file name (these are these two possible problems that I found in previous post related to this topic) Fetching 0 objects´´ when I run Fetching 10 random imgaes from whole experiment. ´♁0 is greater than the number of objects. However, I cannt Fetch images under Classifier. When I run CellProfiler Analyst, it successfully opened the ´´properties file´´, I can open iamges under Image Gallery using Fetch!. Using CellProfiler Analyst for MachineLearning to select the bad quality image. I tried to use similar procedure as published for Quality control (Bray,M.A. Maximal projected images (one plane) and 20 Fields per well. There are 8 channels scanned for each plate. I scanned 6x 96 well plate with Opera Phenix for CellPainting Screening.
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